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wild type mcherryatg3  (Addgene inc)


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    Structured Review

    Addgene inc wild type mcherryatg3
    Under pro-oxidizing conditions a disulfide forms between the catalytic thiols of Atg3 and Atg7. a siRNA knock-down of Atg7 attenuates intermolecular disulfide oxidation of Atg3, and loss of Atg3 prevents Atg7 disulfide dimerization. b Immunoprecipitation of Atg7 leads co-purification of disulfide-bound mCherry-Atg3. c Overexpressed wild-type <t>mCherryAtg3</t> and wild-type Atg7 form a disulfide-bound heterodimer in amino-acid-starved cells treated with H 2 O 2 . However, mutation of the catalytic thiol to an alanine on either Atg3 or Atg7 prevents intermolecular disulfide formation
    Wild Type Mcherryatg3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+mcherryatg3/mCherry-ATG3-C-18+(Plasmid+%2354993)/pmc05758830-264-16-18
    Average 93 stars, based on 2 article reviews
    wild type mcherryatg3 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Oxidation of Atg3 and Atg7 mediates inhibition of autophagy"

    Article Title: Oxidation of Atg3 and Atg7 mediates inhibition of autophagy

    Journal: Nature Communications

    doi: 10.1038/s41467-017-02352-z

    Under pro-oxidizing conditions a disulfide forms between the catalytic thiols of Atg3 and Atg7. a siRNA knock-down of Atg7 attenuates intermolecular disulfide oxidation of Atg3, and loss of Atg3 prevents Atg7 disulfide dimerization. b Immunoprecipitation of Atg7 leads co-purification of disulfide-bound mCherry-Atg3. c Overexpressed wild-type mCherryAtg3 and wild-type Atg7 form a disulfide-bound heterodimer in amino-acid-starved cells treated with H 2 O 2 . However, mutation of the catalytic thiol to an alanine on either Atg3 or Atg7 prevents intermolecular disulfide formation
    Figure Legend Snippet: Under pro-oxidizing conditions a disulfide forms between the catalytic thiols of Atg3 and Atg7. a siRNA knock-down of Atg7 attenuates intermolecular disulfide oxidation of Atg3, and loss of Atg3 prevents Atg7 disulfide dimerization. b Immunoprecipitation of Atg7 leads co-purification of disulfide-bound mCherry-Atg3. c Overexpressed wild-type mCherryAtg3 and wild-type Atg7 form a disulfide-bound heterodimer in amino-acid-starved cells treated with H 2 O 2 . However, mutation of the catalytic thiol to an alanine on either Atg3 or Atg7 prevents intermolecular disulfide formation

    Techniques Used: Knockdown, Immunoprecipitation, Copurification, Mutagenesis

    Related Articles

    Transfection:

    Article Title: Oxidation of Atg3 and Atg7 mediates inhibition of autophagy
    Article Snippet: The pCMV-myc-LC3 ΔC was generated to express a truncated form of human LC3 without the C-terminal 5 amino acids (MKLSV) normally cleaved by Atg4. pCMV-myc-LC3 ΔC was generated from wild-type by introducing a premature stop codon using site directed mutagenesis (QuikChange II XL, Agilent) following the manufacturer’s instructions. .. In some experiments, SMC were transfected for 48 h with a combination of 250 ng of wild-type mCherryAtg3 (Addgene, 54993), C264A mCherryAtg3, wild-type Atg7 (Sino Biological, HG15684) or C572A Atg7 using lipofectamine 3000. .. In other experiments SMC were treated with human oxLDL (Alfa Aesar) in complete growth media for the indicated time.



    Similar Products

    wild type mcherryatg3  (Addgene inc)
    93
    Addgene inc wild type mcherryatg3
    Under pro-oxidizing conditions a disulfide forms between the catalytic thiols of Atg3 and Atg7. a siRNA knock-down of Atg7 attenuates intermolecular disulfide oxidation of Atg3, and loss of Atg3 prevents Atg7 disulfide dimerization. b Immunoprecipitation of Atg7 leads co-purification of disulfide-bound mCherry-Atg3. c Overexpressed wild-type <t>mCherryAtg3</t> and wild-type Atg7 form a disulfide-bound heterodimer in amino-acid-starved cells treated with H 2 O 2 . However, mutation of the catalytic thiol to an alanine on either Atg3 or Atg7 prevents intermolecular disulfide formation
    Wild Type Mcherryatg3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+mcherryatg3/mCherry-ATG3-C-18+(Plasmid+%2354993)/pmc05758830-264-16-18
    Average 93 stars, based on 1 article reviews
    wild type mcherryatg3 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Under pro-oxidizing conditions a disulfide forms between the catalytic thiols of Atg3 and Atg7. a siRNA knock-down of Atg7 attenuates intermolecular disulfide oxidation of Atg3, and loss of Atg3 prevents Atg7 disulfide dimerization. b Immunoprecipitation of Atg7 leads co-purification of disulfide-bound mCherry-Atg3. c Overexpressed wild-type mCherryAtg3 and wild-type Atg7 form a disulfide-bound heterodimer in amino-acid-starved cells treated with H 2 O 2 . However, mutation of the catalytic thiol to an alanine on either Atg3 or Atg7 prevents intermolecular disulfide formation

    Journal: Nature Communications

    Article Title: Oxidation of Atg3 and Atg7 mediates inhibition of autophagy

    doi: 10.1038/s41467-017-02352-z

    Figure Lengend Snippet: Under pro-oxidizing conditions a disulfide forms between the catalytic thiols of Atg3 and Atg7. a siRNA knock-down of Atg7 attenuates intermolecular disulfide oxidation of Atg3, and loss of Atg3 prevents Atg7 disulfide dimerization. b Immunoprecipitation of Atg7 leads co-purification of disulfide-bound mCherry-Atg3. c Overexpressed wild-type mCherryAtg3 and wild-type Atg7 form a disulfide-bound heterodimer in amino-acid-starved cells treated with H 2 O 2 . However, mutation of the catalytic thiol to an alanine on either Atg3 or Atg7 prevents intermolecular disulfide formation

    Article Snippet: In some experiments, SMC were transfected for 48 h with a combination of 250 ng of wild-type mCherryAtg3 (Addgene, 54993), C264A mCherryAtg3, wild-type Atg7 (Sino Biological, HG15684) or C572A Atg7 using lipofectamine 3000.

    Techniques: Knockdown, Immunoprecipitation, Copurification, Mutagenesis